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derivative e g7 ova  (ATCC)


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    ATCC derivative e g7 ova
    Derivative E G7 Ova, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/derivative+e+g7+ova/E%2EG7-OVA/pm42001519-38-4-15
    Average 96 stars, based on 617 article reviews
    derivative e g7 ova - by Bioz Stars, 2026-10
    96/100 stars

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    Expressing:

    Article Title: Antigen-specific T cells fully conserve antitumour function following cryopreservation
    Article Snippet: .. The EL-4 lymphoma cell line and its SIINFEKL expressing derivative E.G7-OVA (cognate antigen-presenting for OT-I) were obtained from American Type Culture Collection and cultured in TCM. ..

    Article Title: Antigen-specific T cells fully conserve antitumour function following cryopreservation.
    Article Snippet: .. The EL-4 lymphoma cell line and its SIINFEKL expressing derivative E.G7-OVA (cognate antigenpresenting for OT-I) were obtained from American Type Culture Collection and cultured in TCM. .. T cells were cryopreserved at day 3 after isolation according to established methods.13 A total of 2–5× 106 T cells were initially pelleted by centrifugation, resuspended in 1 ml of cryopreservation solution made up of 10% dimethyl sulfoxide (Sigma) in foetal calf serum and then transferred into cryogenic vials (Nunc, Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Antigen-specific T cells fully conserve antitumour function following cryopreservation
    Article Snippet: .. The EL-4 lymphoma cell line and its SIINFEKL expressing derivative E.G7-OVA (cognate antigen-presenting for OT-I) were obtained from American Type Culture Collection and cultured in TCM. ..

    Article Title: Antigen-specific T cells fully conserve antitumour function following cryopreservation.
    Article Snippet: .. The EL-4 lymphoma cell line and its SIINFEKL expressing derivative E.G7-OVA (cognate antigenpresenting for OT-I) were obtained from American Type Culture Collection and cultured in TCM. .. T cells were cryopreserved at day 3 after isolation according to established methods.13 A total of 2–5× 106 T cells were initially pelleted by centrifugation, resuspended in 1 ml of cryopreservation solution made up of 10% dimethyl sulfoxide (Sigma) in foetal calf serum and then transferred into cryogenic vials (Nunc, Thermo Fisher Scientific).



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    Significantly fewer CD4 + and CD8 + lymphocytes accumulate in the infected spleens of p47 phox−/− mice during primary L. monocytogenes infection. WT ( ) and p47 phox−/− ( ) mice were infected with 5 × 10 4 CFU (0.1 LD 50 ) rLM-OVA. On the indicated post infection day the mice were euthanized and single-cell splenocyte cultures were incubated for 3–5 h with 1 μ M OVA 323-339 and/or OVA 257-264 peptides for CD4 and CD8 T lymphocyte stimulation, respectively. Monensin was added for the final 2 h of culture, and the cells were harvested and stained for surface CD4, CD8, and intracellular IFN γ expression. ( a ) Percentage of CD4 + and CD8 + lymphocytes, and the percentage of CD4 + and CD8 + lymphocytes expressing IFN γ . The data are the mean (±S.E.M.) for six individual mice from two separate experiments with three of each genotype/experiment. ( b ) p47 phox−/− CD8 + T lymphocytes effectively kill target cells. On post infection days 3 and 7, as indicated, spleen-derived CD8 + T cells were co-cultured with CFSE-labeled <t>EL4</t> or E.G7-OVA target cells at the indicated effect: target cell ratios for 4 h. The results are presented as percentage of PI + E.G7-OVA or EL4 target cells. Results are the mean (±S.E.M.) of three independent experiments including three of each genotype/experiment. ( c ) On post infection day 3, the percentage of non-viable EMA + CD4 + or EMA + CD8 + lymphocytes was determined. The data are the mean (±S.E.M.) percentage for five individual mice. * P <0.005, ** P <0.05
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    Significantly fewer CD4 + and CD8 + lymphocytes accumulate in the infected spleens of p47 phox−/− mice during primary L. monocytogenes infection. WT ( ) and p47 phox−/− ( ) mice were infected with 5 × 10 4 CFU (0.1 LD 50 ) rLM-OVA. On the indicated post infection day the mice were euthanized and single-cell splenocyte cultures were incubated for 3–5 h with 1 μ M OVA 323-339 and/or OVA 257-264 peptides for CD4 and CD8 T lymphocyte stimulation, respectively. Monensin was added for the final 2 h of culture, and the cells were harvested and stained for surface CD4, CD8, and intracellular IFN γ expression. ( a ) Percentage of CD4 + and CD8 + lymphocytes, and the percentage of CD4 + and CD8 + lymphocytes expressing IFN γ . The data are the mean (±S.E.M.) for six individual mice from two separate experiments with three of each genotype/experiment. ( b ) p47 phox−/− CD8 + T lymphocytes effectively kill target cells. On post infection days 3 and 7, as indicated, spleen-derived CD8 + T cells were co-cultured with CFSE-labeled EL4 or E.G7-OVA target cells at the indicated effect: target cell ratios for 4 h. The results are presented as percentage of PI + E.G7-OVA or EL4 target cells. Results are the mean (±S.E.M.) of three independent experiments including three of each genotype/experiment. ( c ) On post infection day 3, the percentage of non-viable EMA + CD4 + or EMA + CD8 + lymphocytes was determined. The data are the mean (±S.E.M.) percentage for five individual mice. * P <0.005, ** P <0.05

    Journal: Cell Death & Disease

    Article Title: PP2A-dependent control of transcriptionally active FOXO3a in CD8 + central memory lymphocyte survival requires p47 phox

    doi: 10.1038/cddis.2012.118

    Figure Lengend Snippet: Significantly fewer CD4 + and CD8 + lymphocytes accumulate in the infected spleens of p47 phox−/− mice during primary L. monocytogenes infection. WT ( ) and p47 phox−/− ( ) mice were infected with 5 × 10 4 CFU (0.1 LD 50 ) rLM-OVA. On the indicated post infection day the mice were euthanized and single-cell splenocyte cultures were incubated for 3–5 h with 1 μ M OVA 323-339 and/or OVA 257-264 peptides for CD4 and CD8 T lymphocyte stimulation, respectively. Monensin was added for the final 2 h of culture, and the cells were harvested and stained for surface CD4, CD8, and intracellular IFN γ expression. ( a ) Percentage of CD4 + and CD8 + lymphocytes, and the percentage of CD4 + and CD8 + lymphocytes expressing IFN γ . The data are the mean (±S.E.M.) for six individual mice from two separate experiments with three of each genotype/experiment. ( b ) p47 phox−/− CD8 + T lymphocytes effectively kill target cells. On post infection days 3 and 7, as indicated, spleen-derived CD8 + T cells were co-cultured with CFSE-labeled EL4 or E.G7-OVA target cells at the indicated effect: target cell ratios for 4 h. The results are presented as percentage of PI + E.G7-OVA or EL4 target cells. Results are the mean (±S.E.M.) of three independent experiments including three of each genotype/experiment. ( c ) On post infection day 3, the percentage of non-viable EMA + CD4 + or EMA + CD8 + lymphocytes was determined. The data are the mean (±S.E.M.) percentage for five individual mice. * P <0.005, ** P <0.05

    Article Snippet: The killing assay was performed by co-culturing CD8 + lymphocytes with CFSE-labeled control EL4 cells (ATCC, Manassas, VA, USA) or the OVA-expressing EL4 derivative cell line E.G7-OVA (ATCC) at the indicated effect: target cells ratios.

    Techniques: Infection, Incubation, Staining, Expressing, Derivative Assay, Cell Culture, Labeling